Advantages of 'Serial Dilutions' This section is not a recipe for your experiment. It explains some principles for designing dilutions that give optimal results. Once you understand these principles, you will be better able to design the dilutions you need for each specific case. Often in experimental work, you need to cover a range of concentrations, so you need to make a bunch of different dilutions.

Serial Dilution Calculations. Example: A stock solution with a concentration of 2.00 ppm was diluted to make three other solutions. For each dilution.

For example, you need to do such dilutions of the standard IgG to make the standard curve in ELISA, and then again for the unknown samples in ELISA. You might think it would be good to dilute 1/2, 1/3, 1/10, 1/100. These seem like nice numbers. There are two problems with this series of dilutions. • The dilutions are unnecessarily complicated to make. Adsl Wireless Modem An1020 25 Software Update. You need to do a different calculation, and measure different volumes, for each one.

It takes a long time, and it is too easy to make a mistake. • The dilutions cover the range from 1/2 to 1/100 unevenly. In fact, the 1/2 vs. 1/3 dilutions differ by only 1.5-fold in concentration, while the 1/10 vs. 1/100 dilutions differ by ten-fold. If you are going to measure results for four dilutions, it is a waste of time and materials to make two of them almost the same.

And what if the half-maximal signal occurs between 1/10 and 1/100? You won't be able to tell exactly where it is because of the big space between those two. Serial dilutions are much easier to make and they cover the range evenly. Serial dilutions are made by making the same dilution step over and over, using the previous dilution as the input to the next dilution in each step. Since the dilution-fold is the same in each step, the dilutions are a geometric series (constant ratio between any adjacent dilutions). For example: 1/3, 1/9, 1/27, 1/81 Notice that each dilution is three-fold relative to the previous one.